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A simple non-enzymatic protocol for isolation and culture of mesenchymal stem cells from adipose tissues
(Nguyen Tat Thanh University, 2018) Nguyen, Thi Phuong; Nguyen, Huu Hung
Mesenchymal stem cells (MSCs) are multipotent stem cells – ability to differentiate into several cell types. Previous studies have showed a high number of these cells can be found in adipose tissue. This study has established a simple protocol for isolation and culture of MSCs from mouse adipose tissue using minimal requirements. With non-enzymatic approach, we successfully isolated and cultured the MSCs. Isolated MSCs were identified through morphology and ability of differentiation into adipocyte-like cells under the presense of stimuli in the culture medium.