An effective protocol for isolation and culture of mesenchymal stem cells from mouse bone marrow

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dc.contributor.authorNguyen, Thi Phuong
dc.contributor.authorNguyen, Huu Hung
dc.date.accessioned2024-08-22T09:23:21Z
dc.date.available2024-08-22T09:23:21Z
dc.date.issued2018
dc.description4 pages.
dc.description.abstractBone marrow is known as the main source of isolated mesenchymal stem cells. This study has demonstrated a protocol for isolation and culture of mesenchymal stem cells from mouse bone marrow (mBM) with minimal requirement. The cells were harvested from the tibias and femurs, and then cultured in Dulbecco’s modified Eagle’s medium supplemented with fetal bovine serum. The mBM-MSCs were isolated by media changing every 8 hours in 24 hours of culture and sub-culture. Target cells subsequently were regconized by adipogenic differentiaton posibility. The study also focused on descript a simple procedure to culture BM-MSCs and the results obtained following the procedure.
dc.identifier.citationNguyen Tat Thanh University. (2018). Journal of Science and Technology - NTTU, No 02. ISSN 2615-9015.
dc.identifier.issn2615-9015
dc.identifier.urihttps://oerrepository.ntt.edu.vn/handle/298300331/111
dc.language.isoen
dc.publisherNguyen Tat Thanh University
dc.subjectBone marrow
dc.subjectCell culture
dc.subjectIsolation
dc.subjectDifferentiation
dc.subjectMesenchymal stem cells
dc.subjectTế bào gốc trung mô
dc.subjectPhân lập
dc.titleAn effective protocol for isolation and culture of mesenchymal stem cells from mouse bone marrow
dc.typeArticle
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