Recombinant protein expression of Magnaporthe oryzae MGG06069 and its polysaccharide monooxygenase domain in Escherichia coli
Các tập tin
Ngày
2022
Tác giả
Bui, Cong Chinh
Ho, Ta Giap
Vu, Van Van
Tên Tạp chí
Tạp chí ISSN
Nhan đề tập
Nhà xuất bản
Nguyen Tat Thanh University
Giấy phép
Tóm tắt
MGG06069 is a polysaccharide monooxygenase protein that originated from the fungus that causes rice blast, Magnaporthe oryzae. It has the potential to speed up the cellulolysis process, which can be beneficial for industry. In this study, the full-length MGG06069 protein and its polysaccharide monooxygenase domain were expressed in Escherichia coli in order to produce recombinant proteins on a large scale for further enzymatic experiment. It was shown that the amount of the recombinant polysaccharide monooxygenase domain protein was much higher compared to that of the full-length protein. In addition, only one band of expected size was obtained for the recombinant PMO domain protein. These results suggest the purified polysaccharide monooxygenase domain of MGG06069 that can be used for enzymatic assays in the future.
Mô tả
5 p.
Từ khóa
AA9 , MGG06069 , Escherichia coli , Polysaccharide Monooxygenase , PMOs , Hoạt tính enzym
Trích dẫn
Nguyen Tat Thanh University. (2022). Journal of Science and Technology - NTTU, (17). ISSN 2615-9015.