Determination of Auramine O in animal feedstuffs using ultra performance liquid chromatography tandem mass spectrometry
Ngày
2019
Tác giả
Nguyen, Thi Ha
Nguyen, Bich Nu
Le, Phuong Thao
Tran, Thi Hong
Nguyen, Kieu Hung
Tên Tạp chí
Tạp chí ISSN
Nhan đề tập
Nhà xuất bản
Nguyen Tat Thanh University
Giấy phép
Tóm tắt
A method based on ultra-high performance liquid chromatography coupled with tandem massspectrometry (UPLC/MS/MS) was developed for simple and rapid determination of the residues of Auramine O in animal feedstuffs. The samples were extracted by MeOH: H2O + HCOOH0.1% and then analyzed in multiple reaction monitoring (MRM) mode. The mobile phase was ultrapure water with 0.1% formic acid. Under the optimized detection conditions, the linear range for Auramine O was 20 - 100µg/L and the linear correlation coefficients found more than 0.99 The limit of quantification of Auramine O was 0.34 mg/kg. The recoveries of Auramine O ranged from 64.71 - 94.12% with relative standard deviations (RSD) of 4.93 - 8.31% with the concentration range of 20 - 100 µg/L. This method is simple, effective, sensitive and is suitable for the determination and confirmation of Auramine O in animal feedstuffs.
Mô tả
6 pages.
Từ khóa
Auramine O , Animal feedstuffs , UPLC/MS/MS
Trích dẫn
Nguyen Tat Thanh University. (2019). Journal of Science and Technology - NTTU, No 06. ISSN 2615-9015.