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Recombinant protein expression of Magnaporthe oryzae MGG06069 and its polysaccharide monooxygenase domain in Escherichia coli
(Nguyen Tat Thanh University, 2022) Bui, Cong Chinh; Ho, Ta Giap; Vu, Van Van
MGG06069 is a polysaccharide monooxygenase protein that originated from the fungus that causes rice blast, Magnaporthe oryzae. It has the potential to speed up the cellulolysis process, which can be beneficial for industry. In this study, the full-length MGG06069 protein and its polysaccharide monooxygenase domain were expressed in Escherichia coli in order to produce recombinant proteins on a large scale for further enzymatic experiment. It was shown that the amount of the recombinant polysaccharide monooxygenase domain protein was much higher compared to that of the full-length protein. In addition, only one band of expected size was obtained for the recombinant PMO domain protein. These results suggest the purified polysaccharide monooxygenase domain of MGG06069 that can be used for enzymatic assays in the future.