An effective protocol for isolation and culture of mesenchymal stem cells from mouse bone marrow
Ngày
2018
Tác giả
Nguyen, Thi Phuong
Nguyen, Huu Hung
Tên Tạp chí
Tạp chí ISSN
Nhan đề tập
Nhà xuất bản
Nguyen Tat Thanh University
Giấy phép
Tóm tắt
Bone marrow is known as the main source of isolated mesenchymal stem cells. This study has demonstrated a protocol for isolation and culture of mesenchymal stem cells from mouse bone marrow (mBM) with minimal requirement. The cells were harvested from the tibias and femurs, and then cultured in Dulbecco’s modified Eagle’s medium supplemented with fetal bovine serum. The mBM-MSCs were isolated by media changing every 8 hours in 24 hours of culture and sub-culture. Target cells subsequently were regconized by adipogenic differentiaton posibility. The study also focused on descript a simple procedure to culture BM-MSCs and the results obtained following the procedure.
Mô tả
4 pages.
Từ khóa
Bone marrow , Cell culture , Isolation , Differentiation , Mesenchymal stem cells , Tế bào gốc trung mô , Phân lập
Trích dẫn
Nguyen Tat Thanh University. (2018). Journal of Science and Technology - NTTU, No 02. ISSN 2615-9015.